PacBio SampleNet is the central resource for information and discussion on sample prep and sequencing with the PacBio System. In addition to downloading the latest PacBio sample prep documents, you can find out what other users are thinking and doing, share your protocols, ask questions and join in the discussion!
Download Pacific Biosciences' latest template prep and sequencing guides, procedures & checklists, and quick reference cards.
The PacBio Sample Preparation Calculator is used to calculate binding and annealing reactions for preparing DNA samples for use on the PacBio RS and PacBio RS II sequencers.
Have a comment or question about a sample prep? We welcome your input in any area of sample prep, including input DNA purification, amplification, target enrichment, fragmentation, SMRTbell™ template preparation, polymerase binding and storage of polymerase-template complex, library quantitation and QC, instrument loading and sequencing, and automation of template prep.
Have you modified a PacBio template prep protocol, or developed your own protocol for preparing samples for the RS? Post your version of any steps in the sample prep process, including shearing, amplification, target enrichment, SMRTbell prep, polymerase binding, and automation.
Are you (thinking of) sequencing an alternate type of template? Would you like to use the RS for a new or different application? Do you have a suggestion for improving the SampleNet site? Make a suggestion, and see if others have thought of it, too. Then you can vote on the ideas you'd like to see implemented.