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Welcome To Shared Protocols

If you have a protocol for any step in the process of preparing samples for the RS system, including your own version of a Pacific Biosciences template prep protocol, we invite you to share it with other users, who may benefit from it and offer their own modifications. Note: protocols on this page are from the community and are not supported by Pacific Biosciences.

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Purification of Contaminated SMRTbellTM Library Using Magnetic Bead Capture

Protocol Owner: kspittle
Created on: Fri May 17 04:22:29 GMT 2013
This protocol provides a method for cleaning up DNA SMRTbell templates from undesired contaminants using a magnetic-bead-based approach. ...More

(0 Reviews)

Guidelines for Preparing Size-Selected ~20 kb SMRTbell Templates

Protocol Owner: PacBio
Created on: Mon Apr 08 21:02:09 GMT 2013
Shared Protocol for preparing ~20 kb SMRTbell™ libraries using the BluePippin™ DNA size selection system ...More

(0 Reviews)

Automated SMRTbell Library Prep on the Biomek FX

Protocol Owner: PacBio
Created on: Mon Mar 11 22:45:50 GMT 2013
Protocols for automation of 10 kb and 2 kb library prep on the Beckman Biomek® FX, developed by Todd Hartley, NCI/SAIC-Frederick ...More

(0 Reviews)

Guidelines for Using PacBio Barcodes for SMRT Sequencing

Protocol Owner: PacBio
Created on: Mon Mar 11 22:29:21 GMT 2013
Guidelines for use of barcodes in multiplexed amplicon sequencing, including a list of the 48 pairs of barcodes designed for the PacBio System, and examples of barcode PCR primers and templates. ...More

(0 Reviews)

Procedure & Checklist - 10 kb to 20 kb Template Preparation and Sequencing

Protocol Owner: PacBio
Created on: Thu Feb 14 18:43:38 GMT 2013
The latest draft very large insert library prep from Pacific Biosciences ...More

(0 Reviews)

Full-Length cDNA Sequencing on the PacBio RS

Protocol Owner: PacBio
Created on: Sun Dec 16 06:17:25 GMT 2012
PacBio recommendations for full-length cDNA synthesis from polyA RNA, and our protocol for converting full-length cDNA into a SMRTbell library. ...More

(0 Reviews)

Biotechniques Report - Direct sequencing of small genomes on the RS

Protocol Owner: PacBio
Created on: Sat Dec 15 00:25:10 GMT 2012
Sanger Institute’s publication on directly sequencing small DNA molecules without standard library preparation. ...More

(0 Reviews)

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SAMPLENET

Welcome To Shared Protocols

Purification of Contaminated SMRTbellTM Library Using Magnetic Bead Capture

Guidelines for Preparing Size-Selected ~20 kb SMRTbell Templates

Automated SMRTbell Library Prep on the Biomek FX

Guidelines for Using PacBio Barcodes for SMRT Sequencing

Procedure & Checklist - 10 kb to 20 kb Template Preparation and Sequencing

Full-Length cDNA Sequencing on the PacBio RS

Biotechniques Report - Direct sequencing of small genomes on the RS

Guidelines for Preparing Size-Selected ~20 kb SMRTbell Templates

Preparing the Plasmidbell Control Complex XL for Sequencing

Biotechniques Report - Direct sequencing of small genomes on the RS

Full-Length cDNA Sequencing on the PacBio RS

Automated SMRTbell Library Prep on the Biomek FX

Guidelines for Using PacBio Barcodes for SMRT Sequencing

Procedure & Checklist - 10 kb to 20 kb Template Preparation and Sequencing